The Agilent InfinityLab 2D-LC Solution with Active Solvent Modulation Achieving Improved Resolution and Sensitivity for Challenging Combinations of Separation Conditions
ثبت نشده
چکیده
A major challenge during method development in two-dimensional liquid chromatography (2D-LC) can be the incompatibility between the first (1D) and second dimension (2D) solvent systems. For challenging combinations of separation systems, volume overload effects such as breakthrough or broad and distorted peaks in the 2D can be observed. Active solvent modulation (ASM) provides a valve-based approach to address this problem, leading to improved resolution and sensitivity in the 2D. This Technical Overview explains the principle of ASM, and demonstrates its effect in the combination of two reversed-phase (RP) LC separations and a combination of hydrophilic interaction liquid chromatography (HILIC) and RPLC. The Agilent InfinityLab 2D-LC Solution with Active Solvent Modulation
منابع مشابه
High-Resolution Sampling 2D-LC Analysis of Mangosteen Pericarp Xanthones Using the Agilent 1290 Infinity II 2D-LC Solution
A high-resolution UHPLC analysis of xanthones present in the extracts of the pericarp of mangosteen (Garcinia mangostana L.) was carried out on an Agilent 1290 Infinity II LC. Excellent resolution and peak capacity (300 calculated at half height) were obtained within a total analysis time, including column reconditioning, of 16.5 minutes. The main xanthone (α-mangostin) was quantified in two ex...
متن کامل2-Dimensional separation of polyphenols in beverages using the Agilent 1290 Infi nity 2D-LC Solution and software-assisted 2D-LC data analysis for comparison of ingredients
This Application Note presents a solution for the analysis of complex samples by an Agilent 1290 Infi nity 2D-LC Solution in combination with dedicated software for data analysis. Polyphenols, inherent in several fruit juices and red wine, are analyzed by comprehensive two-dimensional liquid chromatography (LCxLC). The data are analyzed by recently developed software to compare the constituent ...
متن کاملHigh-Resolution Analysis of Intact Triglycerides by Reversed Phase HPLC Using the Agilent 1290 Infinity LC UHPLC System
The Agilent 1290 Infinity LC System with ultraviolet/visible (UV/VIS) Diode Array detection (DAD) is used to analyze triglycerides in soybean oil under non-aqueous reversed phase gradient conditions. The Agilent 1290 Infinity LC System was used for the chromatographic separation of the sample on 3.0 and 2.1 mm id C18 columns, of various lengths, with 1.8-μm packing materials prepared in 600 bar...
متن کاملDetection of impurities by heart cutting using the Agilent 1290 Infi nity 2 D - LC Solution Application
This Application Note demonstrates the capability of the Agilent 1290 Infi nity 2D-LC Solution for heart-cutting applications. As an example, a minor impurity is resolved by heart cutting from a co-eluting main compound. The minor impurity, which is isolated on a second-dimension column after heart cutting, is verifi ed by additional spiking and statistical evaluation of the separation performa...
متن کاملProfi ling of Citrus Oils and Determination of Furocoumarins in Citrus Oils Using the Agilent 1290 Infi nity 2D-LC Solution
Comprehensive two-dimensional liquid chromatography (LC×LC) using the Agilent 1290 Infi nity 2D-LC Solution with DAD and MS detection is applied for the analysis of citrus oils. The developed method uses normal phase liquid chromatography in the fi rst dimension and reversed-phase liquid chromatography in the second dimension. This combination provides good orthogonality and is valuable for pro...
متن کامل